The Centre of Biophysics of Macromolecules and their Interactions (french acronym: PFBMI) federates an ensemble of state-of-the-art instrumentation for the in vitro molecular-scale characterization of biological macromolecules, together with well established technical and methodological expertise. The mission of the PFBMI is to provide an added value to a great variety of biological projects, enabling the characterization of the intrinsic properties of macromolecules and their assemblies (shape, folding, stability auto-association,…) and of the interactions in which they are involved (stoichiometry, thermodynamic and kinetic parameters, …).
Fields of expertise of the PFBMI :
- Size and shape of macromolecules and their assemblies
- Secondary and tertiary structures of macromolecules
- Thermodynamic stability of macromolecules
- Kinetic and thermodynamic properties of interactions
- Stoechiometry of macromolecular assemblies
Technologies available at the PFBMI :
- Analytical ultracentrifugation
- Circular dichroism
- Dual polarization interferometry (DPI)
- Dynamic light scattering (DLS) cuvette or plate reader
- Fluorescence spectroscopy steady state or stopped flow
- Microcalorimetry (ITC and DSC)
- Microscale Thermophoresis (MST) LabelFree or NT115
- Static light scattering and viscometry
- Surface plasmon resonance (SPR) Biacore T200 and Biorad Proteon
The PFBMI also provides privileged access to instrumentation installed in other research units:
- Differential scanning fluorimetry
- Electrophoretic mobility
- Infrared spectroscopy
- Small-Angle X-ray Scattering (SAXS)
PFBMI equipments are available for the entire scientific community, national and international, but a priority is given to scientists from the Institut Pasteur in Paris and those from the Institut Pasteur International Network.
The PFBMI can welcome projects at any time through a single e-mail portal (firstname.lastname@example.org). A project submission form (upload here) and a sample information form (upload here) must be attached.