Pole
Platform

Imagopole

spencer

Head: Spencer Shorte

Research

The PFID is a research technology platform (core facility) providing expertise and support in optical imaging methods for researchers at the Institut Pasteur. Our activities include service rendering, training, technology-driven research and technology development. They are highly multi-disciplined, and collaborative, with the mission goal focused on the use of quantitative imaging and analysis to understand the processes of cell/tissue-biology, and their usurpation by infection and disease. The R&D is founded upon the need to develop optical imaging methods that bring new understanding of host-pathogen interactions and in situ high-content imaging techniques and their application to infection, cell biology, cellular microbiology, and microbiology. We work on novel techniques extrapolating quantitative information on spatiotemporal dynamics in situ and we push the limits of existing approaches aiming to enhance their performance thereby broadening their experimental utility.

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Members

Topics

Center for Innovation & Technological Research

Development and Application of new Molecular Probes

For High-content imaging, the requisite for adaptation of existing probes and development of new probes is never-ending in the face of new questions and the growing complexity of the processes to be measured. The PFID is therefore committed to development of new molecular probes, and new applications of existing probes, and has several projects running […]

Equipments

Microscope spinning-disk UltraView VOX • Perkin-Elmer • UltraView VOX

Spinning-disk UltraView VOX Inverted microscope, equipped with a Yokagawa CSUX1 spinning- disk, and two cameras to do dual-color simultaneous imaging. It allows the fast acquisition of optically sectioned slices at very high speed, and is compatible with live specimen imaging as it limits phototoxicity. It is equipped with a FRAP module that can do sequential FRAP […]

Microscope spinning-disk UltraView ERS • Perkin-Elmer • UltraView ERS

Spinning-disk UltraView ERS Inverted microscope, equipped with a Yokagawa CSU22 spinning- disk. It allows the fast acquisition of optically sectioned slices at high speed, and is compatible with live specimen imaging as it limits phototoxicity. It is equipped with a FRAP module that can do sequential FRAP stimulation, based on a quick scanner. Features: Available laser […]

Microscope confocal LSM780 ELYRA PS.1 • ZEISS • LSM 780 - Elyra PS.1

Inverted confocal microscope (780 head) coupled with two techniques of super resolution SIM and PALM STORM (Elyra PS.1 head) SR SIM : Super resolution structured illumination PALM/STORM : Photoactivated Localization Microscopy / Stochastic Optical Reconstruction Microscopy It contains also a highly sensitive spectral detector GaAsp of 32 channels. Features :  Available laser lines for excitation […]

Nikon Biostation • Nikon • Biostation IM-Q

Biostation IM-Q The Biostation is a live cell recorder made for very long term imaging of live cells. It is built around an incubation chamber where temperature, CO2 and humidity are controlled as in a standard incubator. A phase contrast objective (40x air) moves below the 35 mm bottom-glass petri-dish (µ-dishes Ibidi, MatTek or the […]

High Content Screening • PERKIN ELMER • OPERA QEHS

High Throughput confocal microscope for fully automated simultaneous high speed and high-resolution image captures.     Specifications Light Sources Laser Violet wavelength 405 nm, power 50 mW Laser Blue: wavelength 488 nm, power 200 mW Laser Yellow: wavelength 561 nm, power 40 mW Laser Red: wavelength 640 nm, power 80 mW External UV unit LED […]

Microscope confocal LSM700 inverted • ZEISS • LSM700 inverted

Inverted microscope, equipped with a laser scanning confocal head. It allows the acquisition of optically sectioned slices. Features : Available light sources 405 nm 488 nm 555 nm 639 nm Halogen lamp HXP Objectives 10X : Pln-Apo 10X/0.45 25X : LCI Pln-Neo 25X/0.8 Imm Korr W-oil-Glyc 40X : EC Pln-Neo 40X/1.3 oil 63X : Pln-Apo […]

Microscope confocal LSM700 upright • ZEISS • LSM700 upright

Upright microscope, equipped with a laser scanning confocal head. It allows the acquisition of optically sectioned slices. Features : Available light sources 405 nm 488 nm 555 nm 639 nm Halogen lamp HBO Objectives 10X : Pln-Apo 10X/0.45 25X : LCI Pln-Neo 25X/0.8 Imm Korr W-oil-Glyc 20X : W N Achroplan 20X/0.5 water “dipping lens” […]

Publications